Fig. 1: AP2 partitions into lipid monolayers enriched in PtdIns(4,5)P2 and TGN38. | Communications Biology

Fig. 1: AP2 partitions into lipid monolayers enriched in PtdIns(4,5)P2 and TGN38.

From: Combined thermodynamic and time-resolved structural analysis of interactions between AP2 and biomimetic plasma membranes provides insights into clathrin-mediated endocytosis

Fig. 1

Simultaneous colocalization of AP2 and PtdIns(4,5)P2 clusters in Langmuir monolayers at the air water interface were reported by fluorescence images of TGN- PtdIns(4,5)P2 monolayer at the air/buffer interface labeled with 1 mol % Bodipy-TMR- PtdIns(4,5)P2 (A) interacting with AP2-labeled with Alexa Fluor 488 (B). Scale bars are 20 μm (main images in A, B) and 10 μm (corresponding insets). Transferred lipid monolayers immersed in buffer were examined by fluid-phase, tapping-mode AFM in absence (C) and presence (D) of AP2. E shows the thickness histograms of samples shown in (C, D) with straight lines being the corresponding Gaussian distributions, respectively. F Time-resolved AFM images shows AP2 yielded an increase of thickness and FWHM after injection until a plateau is reached when AP2 saturates the monolayer. Data points are represented as mean ± S.D. (n = 10). Individual data points can be found in the source data.

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