Fig. 6: OsPIL13 directly regulates OsSAL1 and OsNHX1 gene expression, and enhances drought tolerance to FL-DS.
A Images of WYG7, OE lines of OsSAL1 (OsSAL1-OE), ossal1 mutant, and co-overexpression of both OsSAL1 and OsPIL13 (OsPIL13-OsSAL1) exposed to 20d FL-DS at the graining stage. The vertical bar represents a scale of 10 cm. B Western blot showing OsSAL1 expression levels in WYG7, OsSAL1-OE, ossal1, and OsPIL13-OsSAL1. C, D iWUEFL, and PAP contents in WYG7, OsSAL1-OE, ossal1, and OsPIL13-OsSAL1. Each bar represents the mean of n replicates (n = 10, individual plants) for panel (C) and (n = 4, individual plants) for panel (D). E Stomatal aperture dynamics in WYG7, OsSAL1-OE, ossal1, and OsPIL13-SAL1 during FL treatment. The shapes of the stomatal apertures were marked in red circles. Bar, 10 μm. F Images of WYG7, OsNHX1-OE, and OsPIL13-OsNHX1 exposed to 20d FL-DS at the graining stage and images of WYG7 and OsPIL13-OsNHX1 exposed to 20d DS in the field. The vertical bar represents a scale of 10 cm. G Western blot showing OsNHX1 protein expression levels in WYG7, OsNHX1-OE, and OsPIL13-OsNHX1 under FL-DS. H–J iWUEFL, and yield per plant in WYG7, OsNHX1-OE, and OsPIL13-OsNHX1 under FL-DS. Each bar of data represents the mean of n replicates (n = 10, individual plants) for panels (H–J). I Stomatal aperture dynamics in WYG7, OsNHX1-OE, and OsPIL13-OsNHX1 under FL-DS. The shapes of the stomatal apertures were marked in red circles. Bar, 10 μm. K Summary model representing the regulation of OsPIL13 with two haplotypes on iWUEFL under FL-DS through collaborating with OsSAL1 and OsNHX1. For panels (C, D, H–J), different letters represent significant differences among groups based on one-way ANOVA followed by Tukey’s HSD test (P < 0.05).
