Fig. 1: Strategies to release N-terminus of a POI.

a–c Traditional strategies to remove the N-terminal tag. d An engineered cleavable CPD tag fused to the N-terminus of a POI enables the customization of the first amino acid.

a–c Traditional strategies to remove the N-terminal tag. d An engineered cleavable CPD tag fused to the N-terminus of a POI enables the customization of the first amino acid.