Fig. 4: Reaction optimization and the purification of NbHer2 with a designed Ala at the N-terminus. | Communications Biology

Fig. 4: Reaction optimization and the purification of NbHer2 with a designed Ala at the N-terminus.

From: Facile expression of proteins with desired N-terminal amino acid via an engineered cysteine protease domain

Fig. 4: Reaction optimization and the purification of NbHer2 with a designed Ala at the N-terminus.

The SDS-PAGE analysis of the cleavage activity of Fusion 9 under different temperatures (a), pH (b), and InsP6 concentration (c). SDS-PAGE (d) and LC-MS (e) analysis of the purified A-NbHer2 after cleavage of Fusion 9 induced by InsP6. Lane 1: Marker; Lane 2: A-NbHer2 after purification; Lane 3: reaction system of InsP6-induced cleavage of Fusion 9. The above reactions were performed in 50 mM Tris-HCl.

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