Fig. 5: KMT2D regulates downstream target genes related to synaptic response.
From: Deficiency of KMT2D causes autistic-like behavior in mice and zebrafish
A RNA-seq analysis of zebrafish and mice samples. B Volcano plot showing differentially expressed genes (DEGs) in the km2d+/+ and kmt2d−/− zebrafish. C GO analysis of DEGs in the km2d+/+ and kmt2d−/− zebrafish. D–F Differentially expressed genes associated with glutamatergic and GABAergic synapse in the km2d+/+ and kmt2d−/− zebrafish. D Heatmap of 4 upregulated genes associated with GABAergic synapse, and 6 downregulated genes associated glutamatergic synapse. C RT-qPCR of shank2b (n = 3); D RT-qPCR of gabarapb (n = 3). G Volcano plot showing DEGs in the Km2d-KD and control mice hippocampus. H GO analysis of DEGs in the Km2d-KD and control mice hippocampus. I–K Differentially expressed genes associated with glutamatergic and GABAergic synapse in the Km2d-KD and control mice. I Heatmap of 6 upregulated genes associated with GABAergic synapse, and 6 downregulated genes associated glutamatergic synapse. C RT-qPCR of Cnksr1 (n = 3); D RT-qPCR of Fgf7 (n = 3). Error bars represent as mean ± SD. *P < 0.05, **P < 0.01, Mann-Whitney U test. Mice of Fig. 5, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.
