Table 1 Specific activity of AnGH71B and –C with 100 μM nigerooligosaccharides

From: Structural and biochemical basis for activity of Aspergillus nidulans α-1,3-glucanases from glycoside hydrolase family 71

Enzyme

Nigerotriose

Nigerotetraose

Nigeropentaose

Nigerohexaose

Nigeropentaose-reduced

Nigerohexaose-reduced

AnGH71B

2.50 ± 0.19

391 ± 19

316 ± 20

274 ± 23

16.4 ± 1.6

64.1 ± 8.3

AnGH71C

0.144 ± 0.019

125 ± 7.7

791 ± 110

943 ± 47

230 ± 17

1130 ± 110

  1. Activity (µmol min−1 mgprotein−1) was calculated from linear regression, with associated standard errors, of the rate of oligosaccharide consumption from two independent reactions measured every 2 min over a 10 min period measured by HPAEC-PAD. Reduced oligosaccharides were generated by NaBH4 reduction as described in the methods. Reported values means and standard deviations from duplicate assays (n = 2).
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