Fig. 2: Whole-brain and region-specific dynamics of c-FOS expression during CRC progression.

A The coronal atlas, with the regions of interest highlighted in blue. See Supplementary Table 1 for the full names of brain region. B Quantification of c-FOS positive neurons at two weeks after establishment of CRC mice, comparing Sham mice and CRC mice (n  =  10 to 17 per group, source data provide the number of animals specific to each group). C Quantification of c-FOS positive neurons at three weeks after establishment of CRC mice, comparing Sham mice and CRC mice (n  =  5 to 8 per group, source data provide the number of animals specific to each group). D Representative immunofluorescence images of c-FOS (Green) expression in the anterior cingulate cortex (ACC) at 1, 2, and 3 weeks after MC38 implantation and Sham. E Quantification of c-FOS–positive cells in the ACC over time (n  =  22,8,11,8 per group). F Representative immunofluorescence images of c-FOS (Green) expression in the infralimbic cortex (IL) at 1, 2, and 3 weeks after MC38 implantation and Sham. G Quantification of c-FOS–positive cells in the IL over time (n  =  21,7,15,7 per group). Scale bars: 100 μm. Sham: sham-operated mice, CRC: mice implanted with MC38 cells in the colorectum. Data are presented as mean ± s.e.m. and analyzed by t test with Welch’s correction (B, C). The source data are provided as Supplementary Data 1 in the Figshare repository.