Fig. 1: Acaricide treatment induces ROS accumulation in T. cinnabarinus.
a The O2•− content after fenpropathrin, cyflumetofen and chlorpyrifos treatment in T. cinnabarinus. Acetone was used for the control treatment, and the data was normalized accordingly. Data was expressed as the mean, n = 3. b DHE fluorescence signal intensity in T. cinnabarinus after DHE staining. Acetone was used for the control treatment. Data was expressed as the mean, n = 17–34. In a, b lowercase letters indicate statistically significant differences at different time points following treatment with the same acaricide. In contrast, uppercase letters represent significant differences between different acaricides, as analyzed by two-way ANOVA (Tukey’s HSD), p < 0.05. The F value represents the result of the Fisher test. c T. cinnabarinus were sectioned after DHE staining, and images were obtained using a laser confocal microscope. The scale bar represents 50 μm. The O2•−-specific fluorescent probe is shown in red. In the figure, the deep red signal indicates a higher ROS level.
