Fig. 4: FOXO interacts with CncC, AhR, and HR96. | Communications Biology

Fig. 4: FOXO interacts with CncC, AhR, and HR96.

From: The ROS–FOXO pathway mediates broad-spectrum detoxification of acaricides in Tetranychus cinnabarinus

Fig. 4

a qPCR and Western Blotting analysis of FOXO mRNA and protein expression after dsFOXO feeding. b qPCR analysis of CncC, AhR, and HR96 mRNA expression after FOXO silencing in T. cinnabarinus. In a and b, controls were fed dsGFP and used for normalization. Error bars represent the mean ± SE, n = 3. Asterisks indicate a statistically significant difference analyzed by Student’s t-test. *p < 0.05, **p < 0.01. c FOXO regulates CncC, AhR, and HR96 promoter activities. Fluc/Rluc represents firefly luciferase activity/Renilla luciferase activity. Error bars represent the mean ± SE, n = 3. Lowercase letters represent statistically significant differences, as analyzed by one-way ANOVA (Tukey’s HSD), p < 0.05. d The yeast one-hybrid assay was used to test FOXO binding to the promoters of CncC, AhR, and HR96.

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