Fig. 1: CRISPR/Cas9 screening identifies genetic modifiers of α-syn PFFs accumulation in RPE-1 cells.
From: Novel regulators of heparan sulfate proteoglycans modulate cellular uptake of α-synuclein fibrils
A Strategy of the genome-wide CRISPR screening used to identify genetic modifiers of α-syn-PFFs accumulation in RPE-1 cells. FACS-sorting was used to isolate the cell populations with the 15% lower and 15% higher PFFs fluorescence intensity. B sgRNA enrichment in the Low PFFs population (left panel) and High PFFs population (right panel) was calculated using the MAGeCK algorithm. For all genes in the tKOv3 library, the significance (reproducibility of effect across all 4 sgRNAs for a given gene) was plotted as a function of the maximal Log2 fold change (sgRNA showing the highest enrichment for that gene). The top 20 most significant genes are shown as green or magenta dots, and the associated gene symbols are indicated. C Gene ontology (GO) analysis was performed with the GOrilla online tool, with the ranked lists of genes from the MAGeCK analysis as inputs (Low PFFs population, left; High PFFs population, right). The enriched GO terms for the Process category and their associated false-discovery rate Q-values are reported in the tables.
