Fig. 5: Hif-1α knockdown rescued Pb-induced ferroptosis in astrocyte.

A The AUC score of ferroptosis DEGs following Pb exposure in astrocyte by Augur of sc-RNAseq; B Enrichment score of HIF-1α signalling pathway following Pb exposure; C mRNA expression of Hif-1α in mice brain tissues following Pb exposure; D Gel electrophoresis plot and semi-quantification of HIF-1α in cytoplasm and nucleus of astrocytes followed by different concentrations of PbAc (n = 3); E Content of Fe²⁺ in Pb-exposed astrocytes infected with si-NC and si-Hif-1α (n = 6); F Fe²⁺ visualised by FerroOrange staining in Pb-exposed astrocytes infected with si-NC and si-Hif-1α; G Fluorescence intensity of FerroOrange in Pb-exposed astrocytes infected with si-NC and Hif-1α analysed by ImageJ (n = 3); H GSH, I MDA content in Pb-exposed astrocytes infected with si-NC and si-Hif-1α (n = 6); J Fluorescence peak of LipROS by C11-BODIPY; K The fluorescence intensity of C11-BODIPY in Pb-exposed astrocytes infected with si-NC and si-Hif-1α (n = 6); L Ultrastructure of mitochondria in astrocytes of different groups was imaged by transmission electron microscopy. Red arrowheads: shrunken mitochondria; M Gel electrophoresis plot and semi-quantification of GPX4 and PTGS2 in Pb-exposed astrocytes infected with si-NC and si-Hif-1α; ^*P < 0.05 vs indicated group; ^**P < 0.01 vs indicated group; ^***P < 0.001 vs indicated group. Comparisons are made with ANOVA, followed by Tukey’s multiple comparison tests.