Fig. 5: Functional profile of 1122A11-LALAPG. | Communications Biology

Fig. 5: Functional profile of 1122A11-LALAPG.

From: Structure and function of a cross-neutralizing influenza neuraminidase antibody that accommodates recent N2 NA Asn245 glycosylation

Fig. 5

a 1122A11 and 1122A11-LALAPG were tested in triplicate at 1 μg/mL for binding to increasing concentrations of FcγRI by ELISA. b Increasing concentrations of 1122A11 and 1122A11-LALAPG were tested in triplicate for binding to the recombinant NA protein of A/Hong Kong/2286/2017 by ELISA. Inhibition of recombinant NA (A/Hong Kong/2286/2017) activity was compared between 1122A11 and 1122A11-LALAPG. The recombinant NA was added with serial dilutions of the mAbs, in duplicate, in ELLA (c) and MUNANA (d) assays. Individual technical replicates are presented. e Ab-dependent cellular phagocytosis (ADCP) assay. NA-coated and BSA-coated beads were incubated with hMAb and then added to THP-1 cells. After incubation, cells were assayed for fluorescent bead uptake by flow cytometry. The ADCP score of each hMAb was calculated by multiplying the percentage of bead-positive cells (frequency of phagocytosis) by the mean fluorescence intensity (MFI) of the beads (degree of phagocytosis) and dividing by 106.

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