Fig. 3: THBS1 was upregulated after 5-AC treatment in mouse eyes and hTM cells.
From: Hypomethylation of thrombospondin-1 promoter region is associated with reduced aqueous humor flow
a Cryosections of anterior segments from PBS- (left) and 5-AC-treated (right) eyes were immunostained against THBS1 (green). Nuclei were labeled with DAPI (blue). THBS1 expression in the TM (indicated by yellow arrows) increased after 5-AC treatment when compared to PBS-treated eyes. See also Supplementary Fig. 1. Images are representative of 3 individual mice and were taken using identical settings. Scale bar = 50 µm. CB: ciliary body; TM: trabecular meshwork; SC: Schlemm’s canal. b Relative quantification of real time-quantitative polymerase chain reaction (RT-qPCR) showed that 2-day treatment of 5-AC significantly induced THBS1 gene expression in hTM cells when compared to vehicle-control (n = 3 individual cell strains). For each biological replicate (n), the data point represents the average of three technical replicates of each PCR reaction. Data are represented as mean ± SEM. *P < 0.05 (Student’s t test).
