Fig. 4: Analysis of methylation status of THBS1 promoter in DEX- and vehicle-control hTM cells detected by MSP.
From: Hypomethylation of thrombospondin-1 promoter region is associated with reduced aqueous humor flow
Genomic DNA was bisulfite-treated and amplified with a primer set specifically designed for the THBS1 promoter. MSP results of 3 individual replicates of DEX- (D1-3) and vehicle-control (C1-3) hTM cells are shown. Only unmethylated promoter DNA was detected in samples from DEX treated cells, whereas in control cells both methylated and unmethylated DNA were detected. The original blot is presented in Supplementary Fig. 2C: vehicle-control; D: DEX-treated; L: DNA ladder; M: methylated; U: unmethylated.
