Fig. 4: Erythroblast enucleation was impaired in Pim1^fl/flEpoR^Cre mice.

A Representative flow cytometry analysis and quantitative results of the percentage of CD71⁺Ter119⁺Hoechst33342⁺ cells in peripheral blood of Pim1^fl/flEpoR^Cre mice and controls. N = 6. B Representative cytospin images showing the peripheral blood cells. The red arrow indicating nucleated erythroblasts. C Flow cytometry analyses and statistical results showing the mean of FSC-A, which indicates cell size, of terminal differentiated erythroid cells in Pim1^fl/flEpoR^Cre mice and control mice. D Growth curves of erythroid cells derived from BM lineage^- cells of Pim1^fl/flEpoR^Cre mice and control mice. E Flow cytometry analyses and quantitative results showing apoptotic rate of cultured erythroid cells of Pim1^fl/flEpoR^Cre mice and control mice at 18 hours (h). F, G Flow cytometry analyses and quantitative results showing Ter119⁺CD71⁺ cell percentage of cultured erythroid cells of Pim1^fl/flEpoR^Cre mice and control mice at 18 h and 36 h. H Flow cytometry analyses and quantitative results showing the percentage of enucleated erythroblasts of cultured erythroid cells of Pim1^fl/flEpoR^Cre mice and control mice at 48 h. I Flow cytometry analyses showing the enucleation rate of sorted BM Poly form Pim1^fl/flEpoR^Cre mice and control mice cultured for 18 h and their quantification. Data were presented as mean ± SEM. P values were determined by student’s t test. FSC forward scatter, H33342 Hoechst33342, N.S. no statistic different. *P < 0.05, **P < 0.01, ***P < 0.001.