Fig. 7: PIM1 controlled F-actin network and vesicle trafficking in Ortho.
From: The oncogene protein kinase PIM1 regulates mammalian erythroblast enucleation
A Fluorescence images showing the localization of F-actin in polarized and enucleating Ortho transfected with shRNA-Luciferase or shRNA-PIM1. Scale bar = 10 μm. B Quantitative analysis showing the percentage of enucleating Ortho with concentrated F-actin between nucleus and cytoplasm in PIM1 knockdown groups and controls. C Representative imaging flowcytometry images showing the internalization of CD71 in polarized and enucleating Ortho transfected with shRNA-Luciferase or shRNA-PIM1. D Quantitative analysis showing the percentage of enucleating Ortho with accumulated CD71 between nucleus and cytoplasm in PIM1 knockdown groups and controls. E Fluorescence images depicting the internalization of membrane-impermeable dye FM4-64 in polarized and enucleating Ortho with or without PIM1 deficiency. Scale bar = 10 μm. Data were presented as mean ± SEM. P values were determined by student’s t test. H33342 Hoechst33342, BF bright field. ***P < 0.001.
