Fig. 3: 3D virtual histology of HFOs by parallel-beam synchrotron radiation and correlative imaging.
From: 3D histology of human heart-forming organoids by X-ray phase-contrast tomography
a Light microscopy image of an H& E-stained histological slice of an HFO (HFO Sample 1), correlated to (b) a reconstructed tomographic slice in the xz-plane. Note, that H& E-staining and sectioning was performed after the tomographic recordings. c 3D representation of the reconstructed volume. d Reconstructed tomographic slice in the xy-plane and zoom into the inner core. e Volume rendering of endodermal cavities showing that they are partly connected throughout the IC. f Virtual slice (xz-plane) through a tomographic reconstruction obtained from a different HFO sample (HFO Sample 2) and correlation with an H& E-stained histological slice. The magnified views highlight vessel-like structures (purple and yellow circles) and an endodermal cavity (pink and green circles. g Virtual section through the IC at a different position along the y-direction shows a thin endocardial-like layer, black arrow in the magnified view.
