Fig. 4: 3D virtual histology of HFOs based on laboratory μ-CT.
From: 3D histology of human heart-forming organoids by X-ray phase-contrast tomography
a 3D representation (orthogonal slices) of the reconstructed volume from an HFO (HFO Sample 4) with an effective voxel size of ~ 0.7 μm, a virtual slice through the reconstructed volume and correlative histology (H& E) (registration and visualization with the viewer by Histomography GmbH). The different layers IC, ML, and OL are indicated in the virtual section. The zoom-ins (yellow and blue circles) highlight a vessel-like structure and an endodermal cavity. b Virtual slice through the reconstructed volume resulting from laboratory examination of the same HFO specimen as shown in Fig. 3a–e. The magnified view (green rectangles) shows a sub-region with the three layers of interest IC, ML, and OL. c Virtual section from the tomographic results of the SR-PB setup (cf. Fig. 3d) of the same region as shown in (b).
