Fig. 6: TNAP at the hepatocyte membrane dephosphorylates phosphocholine and phosphoethanolamine.
A Quantification of Pi produced by HuH-6 hepatocytes during the hydrolysis of phosphocholine or phosphoethanolamine, in the presence or absence of TNAPi. B DNA quantification in HuH-6 cells cultured or not with choline and phosphocholine, and in the presence or absence of TNAPi. Results are shown as mean ± SEM from 5 independent experiments for substrate dephosphorylation, and from 3 independent experiments for DNA quantification. For the sake of clarity, individual data points are not presented in (A). However, source data are available in the Supplementary data file. Statistical analysis was performed with a paired t-test.
