Fig. 4: RNA and protein profile following mRNA interactome capture during bovine sperm capacitation.
From: Systematic mRNA interactome analysis reconceptualizes translational quiescence in bovine sperm
A Oligo (dT) beads enriched poly-A RNAs ranging from 500 to 6000 nucleotides in size from total RNA. B Total RNA isolated from bovine sperm before enrichment. C No RNA was associated with unconjugated streptavidin beads (bead control, BC). The peak at 25 nt corresponds to the internal standard (lower marker) to align the ladder data with samples to determine sizing. D Poly-A RNA-associated proteins run on a 3–10% gradient gel and visualized by silver staining showed distinct band patterns in the CAP group compared to C0 and BC groups (E). Western blot analysis (n = 3) validated the presence of one of the RBPs, hexokinase 1 (~103 kDa), in poly-A–associated proteins using an anti-hexokinase 1 antibody.
