Fig. 4: Interferon response C19MC-deleted human trophoblast stem cells (hTSCs).

a hTSCs were first transduced with a lentivector expressing Cas9 under the control of doxycycline, then with a lentivirus expressing two sgRNAs flanking the ATAC-11 element that was previously used to delete this element in BeWo cells. After selection with puromycin, polyclonal cells (dA11) were analyzed by RT-qPCR for selected ISGs and compared to control cells (Cas9). 10 µg/ml poly(I:C) was added overnight. Data are mean ± SD, n = 3. ns not significant, *p < 0.05, **p < 0.01. b RT-qPCR analysis for selected miRNAs in control (Cas9) and edited polyclonal (dA11) hTSCs. 10 µg/ml poly(I:C) was added overnight. Data are mean ± SD, n = 3. ns not significant, *p < 0.05, **p < 0.01. c IFNL1 levels in cell culture medium. Cells were unstimulated or stimulated with 10 µg/ml of poly(I:C) overnight. Supernatants were collected, and the concentration of IFNL1 was determined using ELISA. Data are mean ± SD, n = 3. **p < 0.01.