Fig. 7: AAV expression and Placements Verification.

a Schematic diagram and photomicrographs of CeA where AAV-hSyn-ChR2-eYFP, AAV-DIO-ChR2-eYFP, AAV-hSyn-eYFP, or AAV-DIO-eYFP was microinjected bilaterally staggered around coordinates A/P from Bregma in mm: −2.4, M/L: ±4.1, D/V: −7.835. hSyn, D1 Cre, A2 Cre, and CRF Cre-targeted neurons expressing ChR2-eYFP are shown in green. b Schematic diagram showing surgical placements in hSyn, D1, A2, and CRF ChR2 rats with placement circles color-coded by number of shock rod contacts made by the individual rat corresponding to that site across shock rod test days 1–3 on a color gradient of red (20 contacts) to blue (0 contacts).