Fig. 1: Cryo-EM structures of apo PmtCD in three distinct membrane mimetics. | Communications Biology

Fig. 1: Cryo-EM structures of apo PmtCD in three distinct membrane mimetics.

From: Cryo-EM analysis of the Staphylococcus aureus phenol-soluble modulin exporter PmtCD apo form in detergent micelles, nanodiscs and peptidiscs

Fig. 1

a Cryo-EM reconstructions of apo PmtCD reconstituted in different membrane mimetics, from left to right: detergent LMNG, nanodisc, and peptidisc. The region of the map corresponding to PmtC protomers (nucleotide binding domains) are shown in yellow and orange, and PmtD protomers (transmembrane domains) are shown in blue and red. Predicted membrane span and the PmtCD interfaces therein are indicated between the dashed lines. b In all mimetics, apo PmtCD shows the relatively unusual disposition of the two PmtD promoters completely separated, forming a significant intramembrane lumen which varies somewhat in size. PmtCD model shown as cylinders and coloured as in (a). The intramembrane lumen is denoted by the transparent cyan surface. The lateral entrance and internal cavity dimensions, as well as total volumes, are labelled.

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