Fig. 5: Proposed mechanism of PSM loading by PmtCD. | Communications Biology

Fig. 5: Proposed mechanism of PSM loading by PmtCD.

From: Cryo-EM analysis of the Staphylococcus aureus phenol-soluble modulin exporter PmtCD apo form in detergent micelles, nanodiscs and peptidiscs

Fig. 5

a Dimensions of AlphaFold predicted PSMɑ4 dimer, and x-ray structure (6GQC) of a PSMɑ3 fibril shown in purple cartoon with residues shown as sticks. Heteroatoms are coloured by type (O, red; N, blue; S, yellow). b Nucleotide free PmtCD-peptidisc model shown coloured by hydrophobicity either with a generated surface or cartoon. Hydrophobic residues lining the lumen are shown as sticks and residues chosen for mutational analysis labelled. A PSMɑ4 predicted dimer (purple) is docked into the lumen of PmtCD with residues shown as sticks.

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