Table 1 Cryo-EM data collection and refinement statistics
PmtCD LMNGa | PmtCD Nanodiscb | PmtCD Peptidiscb | PmtCD ADP Peptidiscb | |
|---|---|---|---|---|
Data collection and processing | ||||
Magnification | ||||
Voltage (kV) | 300 | 300 | 300 | 300 |
Electron exposure (e–/Å2) | 50 | 50 | 50 | 50 |
Defocus range (μm) | −1.5 to −3.5 | −1.5 to −3.5 | −1.5 to −3.5 | −1.5 to −3.5 |
Pixel size (Å) | 1.09 | 1.30 | 1.35 | 1.35 |
Symmetry imposed | C1 | C1 | C1 | C1 |
Initial particle images (no.) | 489729 | 1350519 | 614993 | 1580766 |
Final particle images (no.) | 143119 | 143119 | 108661 | 142155 |
Map resolution (Å) | 3.8 | 4.3 | 4.3 | 4.3 |
Refinement | ||||
Initial model used (PDB code) | 6xji | 6xji | 6xji | 6xji |
Model resolution (Å) | 3.8 | 4.3 | 4.3 | 4.3 |
FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
Map sharpening B factor (Å2) | 161 | 172 | 181 | 178 |
Model composition | ||||
Non-hydrogen atoms | 8770 | 8632 | 8632 | 8744 |
Protein residues | 1074 | 1074 | 1074 | 1082 |
Ligands | 2 | 0 | 0 | 2 |
B factors (Å2) | ||||
Protein | 202.32 | 81.89 | 62.99 | 89.26 |
Ligand | 226.26 | 93.74 | ||
R.m.s. deviations | ||||
Bond lengths (Å) | 0.008 | 0.009 | 0.008 | 0.008 |
Bond angles (°) | 1.798 | 1.739 | 1.585 | 1.614 |
Validation | ||||
MolProbity score | 1.61 | 1.60 | 1.51 | 1.64 |
Clashscore | 7.25 | 8.23 | 6.02 | 6.51 |
Poor rotamers (%) | 1.24 | 0.73 | 1.14 | 1.55 |
Ramachandran plot | ||||
Favored (%) | 97.28 | 97.19 | 97.28 | 97.30 |
Allowed (%) | 2.63 | 2.72 | 2.63 | 2.70 |
Disallowed (%) | 0.09 | 0.09 | 0.09 | 0.00 |
