Fig. 5: Spatial transcriptomic analysis of human bone marrow samples.
From: Characterization of the bone marrow architecture of multiple myeloma using spatial transcriptomics
a Experimental workflow for isolation and spatial transcriptomics analysis of healthy and MM human bone tissue samples (created in BioRender. https://BioRender.com/u8jwp6c). b Hematoxylin-Eosin staining (H&E) staining. c Spatial representation of the malignant PC enrichment score. d Spatial gene expression of human PC canonical markers. e CD138 (healthy) and CD38 (human malignant bone tissue (hMM)) IHC, of healthy (upper panels) and human malignant bone tissues (bottom panels). f Anatomical position of the identified areas (Remote zone, Border zone, and Hotspot) in two diseases samples, hMM2 and hMM3. g Box plots per area (mean of all the diseased samples) and spatial distribution of the T cell effector and T cell exhaustion scores. h, i Box plots per area (left panel) (mean of all the diseased samples) and spatial distribution of the scores associated with Neutrophil extracellular trap formation (NETs) (h) and with IL-17 signaling pathways (i) in two human malignant tissues (hMM2 and hMM3) (middle and right panel). Scales of 500 μm (b–i).
