Table 1 Cryo-EM data collection, refinement and validation statistics
#1 Lhcp (EMDB-64036) (PDB 9UC6) | |
|---|---|
Data collection and processing | |
Magnification | 60,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 80 |
Defocus range (μm) | −700 to −2200 |
Pixel size (Å) | 0.859 |
Symmetry imposed | C3 |
Initial particle images (no.) | 5,408,616 |
Final particle images (no.) | 1,291,024 |
Map resolution (Å) | 1.94 |
FSC threshold | 0.143 |
Refinement | |
Initial model used (PDB code) | 8HG5 |
Model resolution (Å) | 2.0 |
FSC threshold | 0.5 |
Map sharpening B factor (Å2) | 65.8 |
Model composition | |
Non-hydrogen atoms | 8379 |
Protein residues | 600 |
Ligands | 69 |
B factors (Å2) | |
Protein | 15.99 |
Ligand | 14.43 |
R.m.s. deviations | |
Bond lengths (Å) | 0.010 |
Bond angles (°) | 1.698 |
Validation | |
MolProbity score | 1.24 |
Clashscore | 4.67 |
Poor rotamers (%) | 0.22 |
Ramachandran plot | |
Favored (%) | 99.00 |
Allowed (%) | 1.00 |
Disallowed (%) | 0.00 |
