Fig. 4: Verification of the interaction between Miro2 and CISD1 in the cells. | Communications Biology

Fig. 4: Verification of the interaction between Miro2 and CISD1 in the cells.

From: Proximity labeling unveils potential roles of the Miro2-CISD1 network in mitochondrial dynamics and neuronal differentiation

Fig. 4

a Top 5 heatmaps of log2 (FC) abundances for Miro2 interactors. b Coimmunoprecipitation of endogenous Miro2 and CISD1 in HCN cells. c Immunofluorescence images of colocalization of Miro2 and CISD1 in HCN cells overexpressing Myc-Miro2. Miro2 was stained with anti-Myc antibody and CISD1 was stained with anti-CISD1 antibody. Yellow areas within merging images indicate that co-localization of Miro2 and CISD1. Nucleus was stained with DAPI. Scale bar: 2 μm. d Immunofluorescence images of SH-SY5Y cells overexpressing Myc-Miro2 and HA-CISD1. Miro2 was stained with anti-Myc antibody and CISD1 was stained with anti-HA antibody. Scale bar: 10 μm.

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