Fig. 1: Differential Transcriptome analysis and functional enrichment of the normal and HIRI groups.
From: STAT1 promotes ferroptosis and inflammation in mouse hepatic ischemia-reperfusion injury
(A) Schematic diagram of the transcriptome sequencing workflow (Created by BioRender); (B, C) PCA analysis of normal and HIRI samples (Sham = 15, HIRI = 15), where the x and y axes represent the two principal components before and after PCA, respectively, with the proportion of explained variance in parentheses; (D) Volcano plot showing the differential expression of 238 DEGs between normal and HIRI samples, with orange representing significantly upregulated genes, yellow dots representing significantly downregulated genes, and gray dots representing genes with no expression difference; (E) Heatmap displaying the top 50 significantly upregulated and downregulated genes, with orange indicating high expression and green indicating low expression; (F) Circular plot illustrating the GO functional enrichment analysis of DEGs, with outer to inner layers representing functional categories, the total number of genes within each category, the number of significantly upregulated genes, and the enrichment factor; (G) Enrichment analysis of DEGs in KEGG pathways, listing the number of genes enriched in each pathway and organizing them into the top six categories of KEGG Pathway; (H) GSVA analysis depicting a heatmap of 171 metabolic pathways in normal and HIRI samples (Sham = 15, HIRI = 15), with red indicating enhanced pathways and blue indicating suppressed pathways; (I, J) Volcano plot and heatmap showing the differential expression of metabolic pathways, with orange indicating significant enhancement and green indicating significant suppression in the volcano plot, and yellow and red indicating significant enhancement and suppression, respectively, in the heatmap; (K−N) Boxplots illustrating the differential feature scores of disease-related pathways, cell signaling and regulation-related pathways, metabolism-related pathways, and cell composition and function-related pathways. The comparison between the two groups is indicated as *P < 0.05, **P < 0.01, and ***P < 0.001.
