Fig. 8: Validation of the effects of STAT1-mediated miR-497-5p/HDAC7 on HIRI Ferroptosis and inflammatory response in vivo.

(A) Gross morphology of the liver in each group, black arrows indicate hepatic congestion, and red arrows denote lobular swelling; (B) Representative H&E stained images of the liver (scale bar: 100 μm/25 μm) and evaluation of liver tissue damage according to Suzuki’s histological criteria. Yellow arrows indicate vacuole formation, and black arrows indicate hepatocellular necrosis; (C−F) Levels of ALT, AST, IL-1β, and IL-6 in peripheral blood of mice; (G−I) Changes in the content of ferroptosis-specific markers MDA, GSH, and SOD in liver cells; (J) Detection of Fe²⁺ content in cells using Prussian blue staining (scale bar: 50 μm), with statistical graph provided. Each group of mice contained 6 individuals, and the values represent the mean ± standard deviation. Comparisons between groups were denoted by asterisks: *P < 0.05, **P < 0.01, ***P < 0.001.