Fig. 8: Effect of Nutlin-3A treatment on T. annulata infected bovine lymphocytes.
From: Genomic characterization of host gene alterations in Theileria annulata-transformed leukocytes
a Immunofluorescence analysis of T. annulata infected cells after 48 hrs treatment with Nutlin-3A using anti-p53 (Green) and TaSP antibody (Magenta). DAPI was used for nuclear staining. b The graph demonstrates the activation of TP53 post Nutilin-3A treatment in T. annulata infected cells. c The box-plot depicts the percentage of dead T. annulata infected cells post Nutilin-3A treatment at 0 hr, 24 hr 48 hr and 72 hr. Error bars represent the mean with standard deviation (SD). Dunnett’s multiple comparison test was applied w.r.t the control group for the treated group at each time point, where p-value < 0.01 was considered to be significant. d Expression levels of AID family genes in T. annulata infected cells: qPCR-based quantification of AID family genes in T. annulata infected cells in comparison to uninfected healthy PBMCs. Error bars indicate the mean ± standard deviation (SD), showing the variation across sample.
