Fig. 1: Schematic of the micov workflow.
From: Calculating fast differential genome coverages among metagenomic sources using micov
Cumulative coverage represents the total coverage breadth achieved when samples are ordered by increasing individual coverage breadth and added sequentially. This display helps assess whether coverage continues to accumulate across a sample group, or instead plateaus early to suggest that only a small part of the genome is represented in the sample. Position plots display coverage patterns across a reference genome. After cumulative coverage plots are generated, a Kolmogorov-Smirnov (KS) test is conducted to quantify differences between chosen sample groups, especially when visual inspection is challenging due to overlapping curves. Key columns of the output include sample groups being compared, KS statistic, and p-value. For genomic region variation, the genome is divided into N bins, and regions are ranked based on the standard deviation (std) of sample hits across groups. Key columns of the output include the ranking, genome id, start and end position of the genomic region, and standard deviation of sample hits (Methods). The figure is created in BioRender. Knight Lab (2024). https://BioRender.com/.
