Table 1 Experimental MINFLUX sequence parameters for 2D single particle tracking of fluorescent dye or QDot-labeled lipid analogs in the SLB
From: Parameter optimization for MINFLUX microscopy enabled single particle tracking
2D Tracking | Pinhole orbit [I] | 1st Pattern iteration [II] | 2nd Pattern iteration [III] |
|---|---|---|---|
L (nm) | 284 | 302 | 150 |
Pattern shape | Hexagon | Hexagon | Hexagon |
Photon limit (counts) | 40 | 20 | 50 (*) |
Laser power factor (times) | 1.0 | 1.5 | 2.0 (*) |
Pattern dwell time (Āµs) | 500 | 100 | 200 (*) |
Pattern repeat (times) | 1 | 1 | 1 |
CFR threshold | ā1.0 | 0.5 | ā1.0 |
Background threshold (kHz) | 15 | 30 | 30 (*) |
MaxOffTime (control param.) | 3āms | 3āms | 3āms |
Damping (control param.) | 0 | 0 | 0 |
Headstart (control param.) | ā1 | ā1 | ā1 |
Stickiness (control param.) | 4 | 4 | 4 |
