Fig. 2: Detection of extracellular trap (ET) formation via immunohistochemistry in swine skin.
From: Injury to tissue caused by device penetration of the skin triggers formation of extracellular traps
ET was the predominant response in the sensor tissue on day 1 and was detectable for 7- and 14-days following sensor insertion. ET levels diminished with the dominance of fibrosis at 21 days after sensor insertion. DAPI staining represents DNA (1×) magnification, with the scale bar representing 1 mm. NETosis was visualized using the DNA stain DAPI (blue) and co-staining with the neutrophil-specific marker elastase (blue) and myeloperoxidase (MPO) (red) at day 1 with magnification (20×), scale bar indicating 100 μm. The stain elastase was replaced with citrullinated histone H3 (green) for weeks 1 to 3.
