Fig. 7: Th2 and Treg cell frequencies in the draining lymph node of AED mice are affected by topical antibiotic exposure in early postnatal life.

The frequency of Th1 (CD4+ IFN-γ+) (a), Th2 (CD4+ IL-4+) (b) and Treg (CD4+ CD25+ Foxp3+) cells (c) in CLNs of naive, OVA and Tob+OVA groups (n = 4–5 per group). Data are shown as mean ± SEM. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 using the one-way ANOVA.