Fig. 3: Immunolocalization of CSM1 and CENH3 in mitotic and meiotic cells.
From: The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms
A–D Mitotic metaphases with Immunolocalization of CSM1 (red) and CENH3 (green). The DNA is stained with DAPI (gray). CENH3 marks the inner kinetochore associated with chromatin, and CSM1 colocalize with CENH3, with a shift toward the cellular poles. This supports the conclusion that CSM1 belongs to the outer kinetochore. Scale bar = 1 µm. E–H Meiotic cell at pachytene, with immunolocalization of CSM1 (red), CENH3 (green) and the cohesin subunit REC8 (blue). REC8 decorates the pair of axis of the synapsed chromosomes. CENH3 marks the kinetochore regions and CSM1 colocalize with it. Scale bar = 3 µm. I–L Meiotic cell at late anaphase I with Immunolocalization of CSM1 (red) and CENH3 (green). The DNA is stained with DAPI (gray). CSM1 colocalizes with CENH3 at the kinetochore Scale bar = 5 µm.
