Table 1 AP-MS of DSN1, CSM1 and KNL1

From: The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms

Bait used for the AP-MS

Number of positive replicates out of three (Affinity Enrichment Score)

Gene name

Protein name or annotation

DSN1-CGSrhino

NGSrhino-DSN1

CSM1-CGSrhino

NGSrhino-CSM1

NGSrhino-KNL1

AT3G27520

DSN1

3 (1043351)

3 (754465)

3 (39715)

3 (107130)

3 (187473)

AT1G61000

NUF2

3 (499278)

3 (390055)

3 (43527)

3 (112944)

3 (214776)

AT3G08880

MUN/SPC24

3 (311870)

3 (255567)

3 (24466)

3 (77961)

3 (114722)

AT4G19350

NNF1

3 (254225)

3 (174948)

3 (16377)

3 (47765)

3 (91088)

AT2G04235

KNL1

3 (220379)

3 (158551)

3 (15598)

3 (65815)

3 (327207)

AT3G54630

NDC80

3 (213395)

3 (164535)

3 (24209)

3 (60327)

3 (95407)

AT3G48210

SPC25

3 (204965)

3 (138528)

3 (12493)

3 (46621)

3 (76219)

AT4G00525

NSL1.1

3 (177567)

3 (134245)

3 (28048)

3 (58279)

3 (112060)

AT5G35520

MIS12

3 (167854)

3 (89767)

3 (12223)

3 (27378)

3 (60705)

AT3G20070

CSM1/TITAN9

3 (121865)

3 (75657)

3 (592365)

3 (539461)

3 (50632)

AT1G01715

NSL1.2

3 (35770)

3 (36498)

  

2 (3484)

AT2G20635

BUB1MAD3/BMF1

3 (32217)

3 (22074)

3 (5278)

3 (19112)

3 (8702)

AT3G24255

ZWINT1.2

3 (21723)

3 (14788)

3 (1173)

3 (4111)

3 (23006)

AT3G23910

ZWINT1.1

3 (7926)

3 (5317)

3 (371)

3 (1273)

3 (9801)

AT1G15660

CENPC

3 (8745)

3 (6291)

3 (2013)

3 (7043)

3 (2401)

AT4G15880

ESD4

3 (14583)

3 (9354)

3 (10052)

3 (13348)

3 (4206)

AT1G19980

-

3 (28223)

3 (25236)

3 (8622)

3 (15805)

3 (12207)

AT1G69400

BUB3,3

3 (13541)

3 (4436)

  

3 (15773)

AT3G10180

CENP-E

3 (3337)

3 (473)

  

3 (2653)

AT3G57000

-

 

3 (119)

 

3 (76)

 

AT2G22230

Thioesterase superfamily protein

3 (107)

    

AT1G17720

Protein phosphatase 2 A

2 (81)

    

AT3G24530

AAA-type ATPase family

 

3 (90)

 

3 (149)

 

AT5G08600

U3 ribonucleoprotein (Utp) family

 

3 (167)

 

3 (94)

 

AT4G10960

UGE5

 

2 (264)

   

AT5G65770

CRWN4

3 (189)

    

AT3G10530

Transducin/WD40 repeat-like superfamily

  

3 (260)

3 (127)

 

AT2G07707

Mitochondrial ATPase

  

3 (738)

  

AT5G43720

rRNA-processing EFG1-like

  

3 (663)

  

AT2G29500

HSP20-like chaperones superfamily

    

3 (2751)

AT4G22740

glycine-rich protein

    

3 (2917)

AT1G03050

AtECA2/PICALM5a

3 (6377)

3 (4258)

   

AT2G44680

CKB4|casein kinase II beta subunit 4

2 (1446)

    

AT5G24630

BIN4, MID|double-stranded DNA binding protein

    

3 (4560)

AT5G25190

ESE3|Integrase-type DNA-binding superfamily protein

    

2 (1467)

AT1G25260

Ribosomal protein L10 family protein

    

3 (376)

AT5G15550

atPEIP2, AtPEP2|Transducin/WD40 repeat-like superfamily protein

    

3 (216)

AT1G13160

ARM repeat superfamily protein

    

3 (196)

AT5G50310

Galactose oxidase/kelch repeat superfamily protein

    

3 (185)

AT4G28250

ATEXPB3, ATHEXP BETA 1.6, EXPB3|expansin B3

    

3 (132)

AT5G06460

ATUBA2, UBA 2|ubiquitin activating enzyme 2

    

3 (122)

AT1G50480

THFS|10-formyltetrahydrofolate synthetase

    

3 (121)

  1. Five AP-MS were performed, each one with three independent purifications followed by mass spectrometry. After filtering, the specifically enriched proteins are reported, with their times identified in three experiments and their enrichment score (=the product of the NSAF ratio and significance (−log(p value)) vs the large dataset. Data are available via ProteomeXchange with identifier PXD062209.