Fig. 2: Interaction between PXN and the envelope protein VP28 of WSSV.

A Protein expression levels of PXN in cell-free hemolymph and hemocytes after WSSV infection. The total protein loading of the cell-free hemolymph was analyzed via SDS-PAGE. Gel staining with Coomassie Brilliant Blue was used as the loading control to verify protein loading amounts for cell-free hemolymph samples, while β-Actin served as the internal control for hemocyte samples. B Interaction between WSSV major proteins (VP15, VP19, VP24, VP26, and VP28) and PXN. C Interaction between VP28 and PXN in cell-free hemolymph and hemocytes. D–G Interaction between rPXN and rVP28. H Colocalization of PXN and VP28 in hemocytes after WSSV infection. Scale bar = 5 μm. For the detection of different proteins (e.g., target protein and internal reference protein), the same protein sample was loaded onto separate gels. After electrophoresis and transfer, different independent blots were obtained.