Table 2 Responses of A. mellifera α5 nAChR mutants expressed in X. laevis oocytes to acetylcholine (ACh) and dopamine (DA)

From: Characterisation of an unusual nicotinic acetylcholine receptor subtype preferentially sensitive to biogenic amines

Mutant

ACh EC50 μΜ

DA EC50 μΜ

DA/ACh response

Wild-type

2072 (773.6–5550)

10.86 (4.409–26.73)

14.94 ± 1.816

E138H

2760 (2386–3193) *p = 0.0382

53.05 (23.16–121.5) *p = 0.0315

9.370 ± 2.033 ****p < 0.0001

V140Q

1410 (672.2–2957) p = 0.7279

30.53 (23.24–40.10) **p = 0.0067

26.54 ± 5.504 **p = 0.0034

S143P

NE

NE

NE

H144P

434.7 (348.3–542.5) *p = 0.0437

Too small responses

0.504 ± 0.118 ****P < 0.0001

S143P + H144P

NE

NE

NE

E138H + S143P + H144P

NE

NE

NE

E138H + V139C + V140Q

+S143P + H144P

NE

NE

NE

human α7lpE

NE

NE

NE

human α7lpC

2055 (1385–3049) p > 0.9999

35.37 (22.09–56.64) p = 0.2925

2.376 ± 0.343 **p = 0.0040

human α7lpC + α7lpE

NE

NE

NE

  1. EC50 values are displayed as the mean (95% confidence limits). DA/ACh response is the mean ± SEM ratio of the maximal responses to 100 μM dopamine and 5 mM acetylcholine. All data are from 5 to 6 different oocytes from ≥3 batches of eggs. NE indicates no expression was detected. ‘Too small responses’ indicates an EC50 curve could not be generated. Statistical test used was one-way ANOVA with Bonferroni’s multiple comparisons test.
  2. *Indicates a significantly different value to that of wild-type (p ≤ 0.05), **(p ≤ 0.01), ***(p ≤ 0.001) and ****(p ≤ 0.0001).
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