Fig. 2: GB13 sensitizes DIPG cells to radiotherapy (RT). | Communications Biology

Fig. 2: GB13 sensitizes DIPG cells to radiotherapy (RT).

From: Radiotherapy plus neoadjuvant and concomitant IL-13Rα2-directed immunotoxin therapy for diffuse intrinsic pontine glioma

Fig. 2

A Clonogenic survival assay conducted on SF8628 cells treated with vehicle control, GB13 at concentrations of 30 pg/mL, 100 pg/mL, or 300 pg/mL GB13 for 24 h prior to RT followed by 48 h post-RT incubation, rinsed with PBS, media changed, and incubated for colony formation. Results are the mean ± SEM of three independent experiments fitted to the linear quadratic (LQ) model. ns: P ≥ 0.05; *: P ≤ 0.05; unpaired t test. B Scanned and digitalized images of a 6-well plate illustrating variable colony formation levels of SF8628 cells subjected to the described treatment regimen: vehicle control, GB13 monotherapy at IC50, 4 Gy of RT alone, or GB13 at IC50 plus 4 Gy RT. Image data were analyzed using ColonyArea software to determine C colony area percentage and D colony intensity percentage6. Results are the mean ± SEM of 3 independent experiments. ns: P ≥ 0.05; **: P ≤ 0.01; ***: P ≤ 0.001; ****: P ≤ 0.0001; two-way ANOVA.

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