Fig. 4: GB13 enhances DNA damage in DIPG cells when combined with RT.
A Representative confocal microscopy images of γH2AX foci in SF8628 cells treated with vehicle control, GB13 alone at IC50, 5 Gy of RT alone, or 5 Gy RT plus GB13 at IC50 (added 24 h pre-RT) for 1 h, 4 h, or 24 h. B Corresponding quantification of γH2AX foci per SF8628 cell for the different treatment conditions and time points (top), and endpoint analysis at 24 h post-RT or GB13 monotherapy (bottom). Results are the mean ± SEM from 3 independent experiments. ns: P ≥ 0.05; ***: P ≤ 0.001; ****: P ≤ 0.0001; one-way ANOVA. For each group, > 300 nuclei were counted. Western blot analysis of the time- and treatment-dependent implications of GB13 alone, RT alone, or RT plus GB13 at IC50 (added 24 h pre-RT) on DNA damage repair and apoptosis induction in (C) SU-DIPG XIII, (D) SU-DIPG XVII, (E) SF8628, and (F) SU-DIPG IV cells. Samples were collected 1 h and 24 h after treatment start. α-Tubulin serves as loading control. Results are representative of 3 independent experiments.
