Fig. 4: Decoding Streptactin-binding plasticity.
From: Decoding protein binding plasticity via integrated deep ribosome display and deep learning
Dimensionality reduction using t-SNE on the latent representations of all available 7-amino-acid sequences in R2 (a) and those with predicted activities over standard Strep-tag (b). c Distribution of predicted values and of the three clusters “H-P”, “E-x-W-L”, and “P-x-W-W-x-x-L”. d Aligned sequence logos of the sequences of three clusters. e Western blot analysis of the pull-down ability of three representative 7-amino-acid peptides, compared to that of the standard Strep-tag peptide. f Fluorescence measurement (left) of the pull-down ability and predicted SBP score of three representative 7-amino-acid peptides, compared to those of standard Strep-tag. (n = 3 biological replicates, bars represent the mean of all replicates with ±SD). 3D Binding diagram of Streptactin with short peptides, HPMGERS (g), SGLELWL(h) and PSWWYSL (i), generated using PyMOL.
