Fig. 4: Relative expression levels of genes surrounding rs113161727 in induced pluripotent stem cell (iPSC)-derived motor neurons from patients with amyotrophic lateral sclerosis (ALS) with each genotype of rs113161727.
From: A genome-wide association study identifies the GPM6A locus associated with age at onset in ALS
The expression levels of genes (GLRA3, ADAM29, GPM6A, WDR17, and SPATA4) surrounding rs113161727 in iPSC-derived motor neurons from 20 patients with ALS were examined using real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR). The expression levels of GPM6A mRNA were significantly higher in iPSC-derived motor neurons with the AG genotype of rs113161727 (n = 10) than in those with the GG genotype (n = 10, p = 0.0039). In contrast, expression levels of the other four genes (GLRA3, ADAM29, WDR17, and SPATA4) were not affected by the rs113161727 genotype. The mRNA expression levels of each genotype were compared using the Mann–Whitney U-test. The mRNA levels of each gene were normalized to the levels of ACTB. RT-qPCR analysis included technical replicates conducted in three wells for each plate. The Cq values from the three technical replicates were averaged to generate a single raw Cq value per assay. Each black dot represents an individual sample. The bottom and top of the box indicate the interquartile range (25th and 75th percentiles), and the line represents the median. The whiskers under and over the box correspond to the minimum and maximum values. Asterisk (*) indicates p < 0.01.
