Fig. 4: EGF receptor family and RET signaling genes in canine thyroid carcinomas.

A–C ERBB2 signaling in canine thyroid carcinomas. A Spearman correlation coefficients (*p < 0.05, **p < 0.01), of GSVA scores derived from ERBB-related gene sets and ERBB2/ERBB4 expression values. B Heatmap of selected pathway GSVA scores and its association with ERBB2, ERBB4 expression levels, ERBB2 copy number variants (CNV) derived from Sequenza bioinformatics resource, thyroid differentiation score (TDS), ERK signature, and MPAS score. The samples were arranged in ascending order of ERBB2 expression levels. C Scatterplot of Spearman coefficients and Log₁₀ FDR derived from the correlation between ERBB2 and 15,015 gene expression levels in FTC samples (*FDR < 0.05). The members of two signaling gene sets are highlighted in two different colors; purple: ERBB2 overexpression to PI3K signaling and magenta: ERBB2 to RAS-ERK signaling. The members common between the two gene sets are highlighted in green. D, E RET signaling in thyroid carcinomas. D Area plot displaying over-expression of seven RET signaling genes in MTC tumors. E The Pearson correlation matrix of genes shows that RET expression was significantly correlated with genes that encode ligands, adaptors, co-receptors, and activators of RET signaling pathway (*p < 0.05, **p < 0.01, **p < 0.001).