Table 1 Sequence of primer pairs used for gene deletion and verification
From: Stevioside curbs Streptococcus pneumoniae infection via inhibiting capsule biosynthesis
Gene | Primer | Sequence (5´-3´) |
|---|---|---|
Upstream | Sense | gtgggaattgctatgggtgc |
Anti-sense | tattttatatttttgttcataatgataactctccttcaatttttttaaac | |
Downstream | Sense | cgggaggaaataattcctctcgccgaaaatcaaatatg |
Anti-sense | ctcaatttctattttaaaaatagctaaggttaacg | |
Erm | Sense | gaaggagagttatcattatgaacaaaaatataaaatattctcaaaac |
Anti-sense | gattttcggcgagaggaattatttcctcccgttaaataatag | |
spxB | Sense | gctcgatgagtttttctaaatgactcaagggaaaattactg |
Anti-sense | catatttgattttcggcgagaggaattatttaattgcg |
