Fig. 5: Heart failure in JPH2_S165F-overexpressing zebrafish embryos. | Communications Biology

Fig. 5: Heart failure in JPH2_S165F-overexpressing zebrafish embryos.

From: S165F mutation-induced Junctophilin-2 autoinhibition impairs Ca2+ signaling in hypertrophic cardiomyopathy

Fig. 5: Heart failure in JPH2_S165F-overexpressing zebrafish embryos.

a Flow diagram of overexpression assays in the zebrafish model. b Representative images of zebrafish embryos at 3 dpf: b1 Sibling; b2 EGFP; b3 Mus_JPH2 WT; b4 Mus_JPH2 S165F; b5 Mus_JPH2 DM. Scale bar: 500 μm. The regions highlighted by dashed boxes are shown at higher magnification; zoom scale bar: 100 μm. Quantification of the area of pericardium/body length (c), body length (d), heart rate (e), and blood flow velocity (f) of 3 dpf zebrafish embryos (n≈20 per group). Data are presented as means ± SD and analyzed using one-way ANOVA compared to the WT group; n.s. not significant, p > 0.05; *p < 0.05; ***p < 0.001; SD standard deviation. g Representative whole-mount in situ hybridization images using nkx2.5 RNA probes. Injected zebrafish embryos at 3 dpf were subjected to in situ hybridization using digoxigenin-labeled: g1 Sibling; g2 Mus_JPH2 WT; g3 Mus_JPH2 S165F; g4 Mus_JPH2 DM. Scale bar: 500 μm. The regions highlighted by dashed boxes are shown at higher magnification; zoom scale bar: 100 μm. h Quantification of the nkx2.5-positive area of 3 dpf zebrafish embryos (n > 20 per group) from assays in (g). Data are presented as means ± SD and analyzed using one-way ANOVA compared to the WT group; n.s. not significant, p > 0.05; ***p < 0.001; SD standard deviation.

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