Fig. 1: Immunolabelling of SpiRab11 in the aboral epithelia of Stylophora pistillata.
From: Insights on the intracellular trafficking of calcifying medium in a reef-building coral
S. pistillata microcolonies growing on glass coverslips were fixed and processed for immunostaining. A, B control sample (incubated with secondary antibody alone); C, D Sample incubated with the anti-HsaRab11 antibody and the secondary antibody. A, C calicoblastic cells; B, D aboral endoderm. Maximum intensity projections of Z-stacks (160 nm sections) acquired through the calicoblastic cells. Grey : Hoechst (nuclei); Magenta: SpiRab11. Maximum intensity projections of Z-stacks acquired through the aboral epithelia. Scale bar 5 µm. Brightfield images of the aboral epithelia are displayed in Supplementary Fig. 4.
