Fig. 5: Analysis of endocytic vesicles containing Lucifer Yellow in the calicoblastic cells of Stylophora pistillata.
From: Insights on the intracellular trafficking of calcifying medium in a reef-building coral
S. pistillata microcolonies were pulsed for 10 min with S-FSW containing 0.5 mg/mL Lucifer Yellow and chased in S-FSW. Colonies were subsequently fixed and immunostained for SOM and Rab11. A mean fluorescence intensity of Rab11 in Lucifer yellow-containing endosomes; B mean fluorescence intensity of SOM in Lucifer yellow-containing endosomes. C proportion of Lucifer yellow-containing endosomes labelled with Rab11. Bars represent mean ± S.D., n = 3 (independent experiments, distinct samples). ANOVA preceded by Levene’s test, followed by Tukey’s post-hoc test, F = 11.84, df = 11; p = 0.0026, * p < 0.05.
