Fig. 1: Nicotine exposure aggravates liver tissue damage in MASH mice.
From: Nicotine exacerbates MASH via inducing intestinal dysbiosis and barrier dysfunction
a Animal model flow chart. b H&E (upper), Masson’s trichrome (middle), and Oil Red O (lower) staining of the liver in mice. Scale bar = 200 μm. c Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mice. d Hepatic triglyceride (TG) content. e Hepatic total cholesterol (TCHO) content. f–i Hepatic gene expression of pro-inflammatory cytokines Il1b, Il6 and Tnf-α, and anti-inflammatory cytokines Il10. j CD20 staining of the liver. Scale bar = 200 μm. k F4/80 staining of the liver. Scale bar = 200 μm. l Ly6G staining of the liver. Scale bar = 200 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: not significant. Data are presented as mean ± SD; n = 6.
