Fig. 5: Antibiotic treatment mitigates nicotine-induced liver damage in MASH mice.
From: Nicotine exacerbates MASH via inducing intestinal dysbiosis and barrier dysfunction
a Schematic of antibiotic cocktail (Abx). b H&E staining of the intestine and liver in mice. Scale bar = 200 μm. c Serum LPS level (n = 6). d Serum ALT and AST levels in mice (n = 6). e Hepatic TG content (n = 6). f Hepatic TCHO content (n = 6). g–i Intestinal gene expression of pro-inflammatory cytokines Tnf-α, Il1b, and Il6 after Abx treatment (n = 6). j–l Gene expression of ZO-1, occludin, and claudin-1 (n = 6). m–p Western blot analysis for ZO-1, occludin, and claudin-1 normalized by β-actin (n = 3). q Changes in relative mRNA levels of genes related to intestinal barrier according to results of gene sequencing of intestinal tissues. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are presented as mean ± SD.
